Visual detection by the rod system in goldfish of different sizes

Abstract

New rods are continually generated and inserted across the entire differentiated retina in juvenile and adult goldfish; no other retinal cells share this characteristic. How does the preferential addition of rods affect visual function? To examine the relation between continued rod addition and visual sensitivity, we measured absolute threshold in fish of different sizes. Twenty-nine fish were trained in a classical conditioning paradigm and psychometric functions were obtained for each of them for detection of a 532 nm light 5 sec in duration, 140 deg in angular subtense, presented while the fish was fully dark adapted. We found that absolute threshold (expressed in terms of retinal photon density) was lower in larger fish, but by a very small amount: on average, large fish (15.4 ± 0.5 cm standard body length) were 1.45 times more sensitive than small fish (4.3 ± 0.3 cm). Morphometric analysis showed that the planimetric density of rods in goldfish retina increases at a similar rate between small and large fish, while the density of retinal ganglion cells declines between small and large fish (by a factor of 3.8). The ratio of rods to ganglion cells (a possible indicator of neural convergence) increased, but by a factor that is too large to reconcile with the psychophysical results (5.3 ×). The results suggest that absolute visual threshold in the goldfish is closely related to the density of rods in the retina.