Visual Construction of Logical AND and NAND Gates

Xiyuan Wang,Zhen Tang,Jianzhong Cui,Zhixiang Yin,Jing Yang,Rujie Xu

doi:10.1155/2022/1319762

Abstract

DNA logic gates are an important branch of DNA computing and have a wide range of applications in DNA computing. In this study, logic circuits of AND gate and NAND gate are built on origami substrate. The realization of AND gate uses polymerase strand displacement (PSD) reaction and hybridization chain reaction (HCR). If there is a fluorescent band “1” displayed, the result is true. The realization of the NAND gate requires a cyclic reaction. If there is a fluorescent band “A” or “T” displayed, the result is true; if no fluorescent band is displayed, the result is false.

Highlights

Computer technology is considered to be one of the three major scientific revolutions in the 20th century, and electronic computers have played a huge role in promoting social development
In the design of the NAND gate, the hairpins with two kinds of fluorescence are anchored on the origami substrate to form two kinds of fluorescent bands “A” and “T,” respectively, and the initiation strand is set as a variable
Visualization AND gate and NAND gate models are constructed on origami based on polymerase strand displacement (PSD) reaction and cyclic reaction

Summary

Introduction

Computer technology is considered to be one of the three major scientific revolutions in the 20th century, and electronic computers have played a huge role in promoting social development. Since DNA origami and DNA logic circuits are both important branches of DNA computing, researchers began. Journal of Chemistry to consider combining both, namely, studying logic circuits on DNA origami and achieved fruitful results [20–30]. A visual AND gate and a NAND gate model are designed on the DNA origami platform. In the design of the AND gate, the hairpins with fluorescence and fluorescence quencher are anchored on the DNA origami platform to form a “1” structure, and the initiation strand and polymerase are set as variables. In the design of the NAND gate, the hairpins with two kinds of fluorescence are anchored on the origami substrate to form two kinds of fluorescent bands “A” and “T,” respectively, and the initiation strand is set as a variable.

Principle of DNA Strand

Principle of Polymerase Strand Displacement Reaction

Principle of Hybridization Chain Reaction

Building

Cyclic Reaction of NAND Gate

Conclusion