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Abstract
BackgroundVISPR is an interactive visualization and analysis framework for CRISPR screening experiments. However, it only supports the output of MAGeCK, and requires installation and manual configuration. Furthermore, VISPR is designed to run on a single computer, and data sharing between collaborators is challenging.ResultsTo make the tool easily accessible to the community, we present VISPR-online, a web-based general application allowing users to visualize, explore, and share CRISPR screening data online with a few simple steps. VISPR-online provides an exploration of screening results and visualization of read count changes. Apart from MAGeCK, VISPR-online supports two more popular CRISPR screening analysis tools: BAGEL and JACKS. It provides an interactive environment for exploring gene essentiality, viewing guide RNA (gRNA) locations, and allowing users to resume and share screening results.ConclusionsVISPR-online allows users to visualize, explore and share CRISPR screening data online. It is freely available at http://vispr-online.weililab.org, while the source code is available at https://github.com/lemoncyb/VISPR-online.
Highlights
VISualization of crisPR screens (VISPR) is an interactive visualization and analysis framework for CRISPR screening experiments
To better help users explore CRISPR screening results, we developed VISPR (VISualization of crisPR screens), an interactive visualization program as part of MAGeCK-VISPR
The gene summary file and normalized count file are mandatory for MAGeCK and MAGeCK-VISPR
Summary
VISPR is an interactive visualization and analysis framework for CRISPR screening experiments. It only supports the output of MAGeCK, and requires installation and manual configuration. Results: To make the tool accessible to the community, we present VISPRonline, a web-based general application allowing users to visualize, explore, and share CRISPR screening data online with a few simple steps. Apart from MAGeCK, VISPR-online supports two more popular CRISPR screening analysis tools: BAGEL and JACKS. It provides an interactive environment for exploring gene essentiality, viewing guide RNA (gRNA) locations, and allowing users to resume and share screening results. Conclusions: VISPR-online allows users to visualize, explore and share CRISPR screening data online.
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