Abstract
The detection of bacterial growth in liquid media is an essential process in determining antibiotic susceptibility or the level of bacterial presence for clinical or research purposes. We have developed a system, which enables simplified and automated detection using a camera and a striped pattern marker. The quantification of bacterial growth is possible as the bacterial growth in the culturing vessel blurs the marker image, which is placed on the back of the vessel, and the blurring results in a decrease in the high-frequency spectrum region of the marker image. The experiment results show that the FFT (fast Fourier transform)-based growth detection method is robust to the variations in the type of bacterial carrier and vessels ranging from the culture tubes to the microfluidic devices. Moreover, the automated incubator and image acquisition system are developed to be used as a comprehensive in situ detection system. We expect that this result can be applied in the automation of biological experiments, such as the Antibiotics Susceptibility Test or toxicity measurement. Furthermore, the simple framework of the proposed growth measurement method may be further utilized as an effective and convenient method for building point-of-care devices for developing countries.
Highlights
A bacterial culture is one of the most basic and core experimental method used for various applications in research, clinical, and industrial fields
As the bacteria grew in the culture tube, the spectrum area of the FFT image decreased, making
As the bacteria grew in the culture tube,broth the spectrum area for of the image decreased, making were measured by taking 1 mL of culture in the cuvette the FFT
Summary
A bacterial culture is one of the most basic and core experimental method used for various applications in research, clinical, and industrial fields. Many frequently performed experiment protocols, such as plasmid transformation, require growth of bacteria until the OD value of the broth reaches a certain value Most of these OD measuring devices are very costly which can be inappropriate for the clinics or laboratories in developing countries where bacterial infection outbreaks are frequent. These sensitive devices are not appropriate for on-site operation where a low-cost growth detection system might be required. These devices can cause issues in maintenance as the current systems require intensive maintenance procedures, including calibration and alignment of the light sensors
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