Abstract
Photochemical and enzymatic synthesis of formic acid was investigated from HCO 3 - with formate dehydrogenase (FDH) from Saccharomyces cerevisiae and reduced methylviologen (MV 2+ ) produced by the visible light photosensitization of zinc tetrakis(4-methylpyridyl) porphyrin (ZnTMPyP) in the presence of triethanolamine (TEOA) as an electron donating reagent. Irradiation of a solution containing TEOA, ZnTMPyP, MV 2+ , NaHCO 3 and FDH in potassium phosphate buffer, with visible light resulted in formic acid production and HCO 3 - consumption. The optimum FDH activity was 20 units and the amount of formic acid and the yield of HCO 3 - to formic acid after 4 h irradiation were estimated to be 50 μmol.dm -3 and 5.0%, respectively.
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