Abstract
Nanoparticle-based drug delivery systems for cancer therapy offer a great promising opportunity as they specifically target cancer cells, also increasing the bioavailability of anticancer drugs characterized by low water solubility. Platicur, [Pt(cur) (NH3)2](NO3), is a cis-diamine-platinum(II) complex linked to curcumin. In this work, an ultrasonication method, coupled with layer by layer technology, allows us to obtain highly aqueous stable Platicur nanocolloids of about 100 nm. The visible light-activated Platicur nanocolloids showed an increased drug release and antitumor activity on HeLa cells, with respect to Platicur nanocolloids in darkness. This occurrence could give very interesting insight into selective activation of the nanodelivered Pt(II) complex and possible side-effect lowering. For the first time, the metabolic effects of Platicur nanocolloid photoactivation, in the HeLa cell line, have been investigated using an NMR-based metabolomics approach coupled with statistical multivariate data analysis. The reported results highlight specific metabolic differences between photoactivated and non-photoactivated Platicur NC-treated HeLa cancer cells.
Highlights
Over the last decade, radiation, chemotherapy, and surgery have become the typical cancer treatment protocols in oncological patients.[1−3] On the other hand, all three methods risk damaging normal tissues or lead to incomplete eradication of the cancer
Chitosan−pectin polysaccharides were chosen as Platicur delivery systems obtaining Platicur nanocolloids of about 100 nm, by ultrasonication-assisted layer by layer technology (LbL)
A 1H NMR metabolomics approach was used to detect in depth the metabolic profile of the HeLa cancer cell line treated with light-activated Platicur NCs
Summary
Radiation, chemotherapy, and surgery have become the typical cancer treatment protocols in oncological patients.[1−3] On the other hand, all three methods risk damaging normal tissues or lead to incomplete eradication of the cancer. Nutrient substrates, such as amino acids (isoleucine, valine, leucine, tyrosine, histidine, lysine, glutamine, phenylalanine, alanine, glycine, and glutamate) and glucose, the essential elements for cell growth, characterize the culture medium NMR spectra. At the same time of drug exposure, HeLa cells show selective consumptions of nutrients in response to growth needs and according to the different drug exposures, indicating different cellular responses and different adaptive mechanisms
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