Viscosity-reducing and Biosurfactant-producing Bacterial Consortia Isolated from Low-permeability Reservoir in Ordos Basin

Abstract

Many bacteria have been proved to change physical (viscosity, wettability, and tension), and compositions of crude oil, which can make it easier for oil to be released from rock pores and achieve the purpose of improving recovery, which is called Microbial Enhanced Oil Recovery (MEOR). Our team has previously isolated six emulsified and viscosity-reducing bacteria (Bacillus. sp.) in low permeability layers (Chang 4+5 and Chang 6) of Ordos Basin. However, environmental tolerance of the strains is limited, and the components of crude oil used by the strains were also different. The combination of strains of different species and genera may enhance the effects of single bacteria, surpass the tolerance upper limit, and optimize the viscosity reduction and degradation. Therefore, in this study, it is extremely necessary to study the bacterial consortium. Two consortia were obtained and each consortium consisted of three bacterial strains and was designated as Consortium A (51+61, 61+H-1, 51+H-1; A-ALL) and Consortium B (34(2)+42, 34(2)+A-3; 42+A-3. B-BLL). The performance of the mixed strains was evaluated by the analysis of change in emulsification rate, crude oil composition, viscosity, and the tolerance (temperature, salinity, and pH) though GC-MS, rotational rheometer, and other methods. The results showed that bacterial consortiums had higher alkali resistance and could survive temperatures of 55 °C and salinity of 50 g/L in comparison to single bacterium. The emulsification rate was 22%-48%. Consortium B has better effects than Consortium A. The viscosity reduction rate of the Consortium A after 7 days was exceeded 30% as a whole, and the rate of Consortium B was more than 35%. The crude oil of Consortium B is basically non-stick to flask. Compared with single bacteria, the utilization components of crude oil to bacteria are still different, including both long chain hydrocarbons and short chain hydrocarbons. However, the proportion of long chain n-alkanes is further reduced compared with that of single bacteria, and the highest ratio is reduced by 23.81% (B-ALL). Overall, the bacterial consortium outperforms the single strain in terms of tolerance, viscosity reduction, and degradation, which further optimizes the application of MEOR.