Abstract
The TCID 50 (50 per cent tissue culture infective dose) method was found to be superior to the PFU (plaque forming unit) method for the detection and evaluation of viruses in sewage. Parallel statistical testing of the two-phase polymer separation method and of the alginate ultra-filtration method for the quantitative concentration of viruses in water samples showed comparable concentration and recovery of virus. These methods of concentration proved effective in the testing of the virus removal by the advanced waste-water treatment plant at Windhoek. Although enteroviruses and reovirus entered the sewage purification plant at levels as high as 20,000 TCID 50 per litre, no virus could be recovered from the finally treated water.
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