Abstract
The rates of adsorption of 32P-labelled fowl plague virus and 131I-labelled vaccinia virus by monolayers of chick embryo and HeLa cells have been investigated. With both types of virus and cell the observed rates of adsorption were very nearly one half the rates expected from Brownian theory and observed when virus was adsorbed by glass and other non-biological surfaces. The adsorption of viruses by cells is not depressed by protein. Adsorption of viruses by cells is dependent on the concentration of cations in the medium, and is depressed by high concentrations of multivalent cations. The rate of adsorption is unaffected by heat inactivation, weak formalin treatment and thiol reagents. The rate of adsorption is increased by low concentrations of polycations and in the presence of high concentrations of uranyl ions; it is diminished by low concentrations of polyanions and by acetylation of the amino groups of the virus. It is suggested that the main interacting groups are the amino groups of the virus and phosphate groups of the host cell wall. The effects of ions on adsorption cannot be explained by current theories describing the interaction of charged surfaces.
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