Abstract
Leukemic and normal mice of the AKR/J strain, infected with Gross leukemia virus, were examined for electron microscopy using the freezeetch technique. The leukemic mice all had lymphocyte counts in excess of 25,000/mm3 and hyperplastic lymph nodes, spleen and thymus.The lymphatic tissues from normal and leukemic animals were strained through a fine fiberglass screen into phosphate-buffered saline at 4°C. The cells were centrifuged at 200XG at 4°C, the pellet was resuspended in phosphate-buffered saline with 25% glycerol and allowed to incubate in the glycerol solution for two hours before final centrifugation at 500XG. The glycerinated specimen was first frozen in Freon 22 and finally in liquid nitrogen. Freeze etching was carried out in a Denton apparatus at a pressure of 106 TORR and 100°C for one minute.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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