Virus-Free Shoots from Cassava Stem Cuttings Infected with Cassava Latent Virus

Abstract

whiteflies were killed with malathion Pacumbaba, R. P. 1985. Virus-free shoots from cassava stem cuttings infected with cassava latent sprays after 8 hr of feeding on the test virus. Plant Disease 69:231-232. plants. Ten and 15 symptomless, unsevered Cassava stem cuttings infected with cassava latent virus (CLV) gave rise to shoots that showed CLV shoots in the first and second year of the symptoms at emergence, shoots that showed CLV symptoms 3-5 wk later, and shoots that remained symptomless after 6 wk of observation. CLV symptoms appeared 5-11 days earlier when experiment, respectively, were also symptomless shoots were detopped than when they were left intact. Symptomless shoots severed 2 detopped the third week after shoots wk after emergence from CLV-infected stem cuttings and subsequently detopped did not develop emerged from the ground. An equal CLV symptoms. These plants, however, readily developed symptoms of CLV when colonized with number of unsevered symptomless shoots CLV-laden Bemisia tabaci. Therefore, symptomless shoots were considered free of CLV and could that were not detopped served as be used as healthy planting stock. controls.