Virus enzyme-linked cell immunoassay (VELCIA): Detection and titration of rotavirus antigen and demonstration of rotavirus neutralizing and total antibodies

Abstract

Virus enzyme-linked cell immunoassay (VELCIA) for detection and titration of rotavirus antigen has been developed. Wild-type porcine rotavirus antigen can be detected and titrated directly from the fecal material within 24 h. Porcine OSU strain can be titrated higher than 10 −8. The method has also been introduced for the demonstration of rotavirus neutralizing and total antibodies. In VELCIA the advantages of the cell culture system for virus isolation are combined with enzyme immunodetection and spectrophotometrical reading of the test.