Abstract
Virus-like leaf symptoms and dieback were observed on elm trees in a public park near Potsdam. Infection with Cherry leaf roll virus (CLRV), Elm mottle virus (EMV), Arabis mosaic virus (ArMV) and Tobacco ringspot virus (TRSV), well known viruses to infected elm trees was excluded by bioassays and serological tests. Poty- or carlavirus- like flexible particles of approximately 750 nm in length were isolated repeatedly from diseased elms. The particles were transmissible to diverse Chenopodium species, a herbaceous indicator. The virus was not a member of the Potyviridae family, based on an ELISA and an RT-PCR assay using a potyvirus genus-specific broad-spectrum polyclonal antibody and family-specific primers, respectively. Also no potyvirus-like pinwheel inclusions were found in leaf cells of infected indicator plants in electron microscopic studies. Further molecular characterization of these virus isolates is under way.
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