Abstract
Crocidolomia pavonana is an important pest of broccoli plants. Pest control is currently still using chemical pesticides that negatively impact the environment. It is hoped that Beauveria bassiana will become one of the environmentally friendly bioinsecticides. The research aimed to study the virulence level of conidia density of B. bassiana as a bioinsecticide against C. pavonana pests on broccoli plants. This experiment used a completely randomized design consisting of 5 treatments and four replications so that 20 experimental units were obtained. The Basic Plant Protection Laboratory and Plant Pest Science Laboratory Faculty of Agriculture, Syiah Kuala University as the research location, which was carried out from January to September 2021. The parameters observed were larval mortality, pupae formed, imago appeared, an incubation period of B. bassiana conidia, and average time of C. pavonana. The results showed that B. bassiana with conidia density of 108/mL distilled water was effective as a bioinsecticide against C. pavonana. The highest percentage of C. pavonana mortality observed at 6 Days After Application (DAA) occurred at a density of 108 conidia/mL of water (97.50%), and the lowest was at a density of 102 conidia/mL of distilled water (62.50%). The percentage of pupae formed was observed. At 7, DAA was 15% using a density of 102 conidia /mL aquadest. Produce 5%, a density of 104 conidia/mL of distilled water was used. The percentage of imago that appeared with a conidia density of 102/mL of distilled water killed at 14 DAA yielded 15%, and 5% resulted from a density of 104 conidia/mL of distilled water. The fastest incubation occurred at a density of 108 conidia/mL of distilled water with a time of 3.50 days. Death of C. pavonana at 3.03 days required conidia density of B. bassiana 102/mL of distilled water.
Highlights
Crocidolomia pavonana is an important pest on vegetable crops, such as broccoli and other vegetables [2]
The results of observations on the mortality of C. pavonana larvae due to the application of B. bassiana conidia with different density levels in each treatment, and observed at 3 to 6 days after application (DAA) showed that the effect was very significant at each observation time, namely 3, 4, 5 and 6 days after application of conidia B. bassiana
The results showed that the density of conidia aquadest B. bassiana 108/mL had a positive effect on its virulence against C. pavonana pests
Summary
Crocidolomia pavonana is an important pest on vegetable crops, such as broccoli and other vegetables [2]. This pest destroys broccoli plants in groups. The initial attack on the growing point of the leaf, all the leaves are eaten, leaving only the leaf bones. Heavy attacks from this pest result in economic losses [15]. It is necessary to control other effective and environmentally friendly methods, one of which is through the use of biological agents [13]. The fungus B. bassiana is a biological agent that can be used as a bioinsecticide against insect pests from several insect orders, such as Lepidoptera, Received: September 2021| Revised: February 2022 | Accepted: February 2022
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