Abstract
Aim:The aim was to detect virulence gene associated with the Salmonella serovars isolated from pork and Slaughterhouse environment.Materials and Methods:Salmonella isolates (n=37) used in this study were isolated from 270 pork and slaughter house environmental samples collected from the Ahmedabad Municipal Corporation Slaughter House, Ahmedabad, Gujarat, India. Salmonella serovars were isolated and identified as per BAM USFDA method and serotyped at National Salmonella and Escherichia Centre, Central Research Institute, Kasauli (Himachal Pradesh, India). Polymerase chain reaction technique was used for detection of five genes, namely invA, spvR, spvC, fimA and stn among different serovars of Salmonella.Results:Out of a total of 270 samples, 37 (13.70%) Salmonella were isolated with two serovars, namely Enteritidis and Typhimurium. All Salmonella serovars produced 284 bp invA gene, 84 bp fimA and 260 bp amplicon for enterotoxin (stn) gene whereas 30 isolates possessed 310 bp spvR gene, but no isolate possessed spvC gene.Conclusion:Presence of invA, fimA and stn gene in all isolates shows that they are the specific targets for Salmonella identification and are capable of producing gastroenteric illness to humans, whereas 20 Typhimurium serovars and 10 Enteritidis serovars can able to produce systemic infection.
Highlights
Pork is one of the most widely eaten meats in the world, accounting for about 38% of meat production worldwide, consumption varies widely from place to place [1]
Presence of invA, fimA and stn gene in all isolates shows that they are the specific targets for Salmonella identification and are capable of producing gastroenteric illness to humans, whereas 20 Typhimurium serovars and 10 Enteritidis serovars can able to produce systemic infection
All 37 Salmonella isolates (13 of which belonged to serovar Enteritidis and 24 belonged to Typhimurium) contained the invasion gene invA, other studies having reported similar results [17,21,22,23,24], which was expected since the invA is an invasion gene conserved among Salmonella serotypes
Summary
Pork is one of the most widely eaten meats in the world, accounting for about 38% of meat production worldwide, consumption varies widely from place to place [1]. The present production of meat in India is estimated at 6.27 million tons in 2013 [2], which are 2.21% of the world’s meat production. The meat production has increased from 764,000 tons in 1970-71 to 6.27 million tons in 2010 in India, which is 2.21% of the world’s meat production. According to the Food and Agriculture Organization of the United Nations, world’s pork production reached 114.2 million tons in 2012. Asia is the principal region, accounting for almost 60% of world pig meat production, World meat production is anticipated to expand modestly in 2013 to reach 308.3 million tons, an increase of 4.2 million tones or 1.4%, compared with 2012 [3]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
