Abstract
Vaginitis is one of the most common reasons women visit a gynecologist. Escherichia coli has been isolated from women with vaginitis, but its role as a vaginal infection aetiological agent is controversial. This study aimed to detect virulence genes and determine the antimicrobial susceptibility of E. coli strains isolated from monomicrobial and polymicrobial cultures collected from women with vaginitis. The presence of the following virulence genes: papC, hly, iucC, afa, fimH, neuC, sfa/foc, cnf1, usp, and ibeA in two E. coli groups was determined by PCR. The antibacterial susceptibility of strains was tested. A higher percentage (93.3%) of isolated strains from monomicrobial cultures with virulence genes in relation to polymicrobial cultures (56.7%) was found. The most frequent virulence genes in both groups were hly (p = 0.0357), fimH (p = 0.000), and cfn1 (p = 0.000). In addition, E. coli isolated from monomicrobial cultures showed 5 genetic combinations compared to the 10 observed in the polymicrobial cultures. An increased number of strains were sensitive to cefotaxime, moxifloxacin, and ciprofloxacin. A high resistance to trimethoprim-sulfamethoxazole was observed. Most of the E. coli strains isolated from monomicrobial cultures and some from polymicrobial cultures showed virulence genes. A better understanding of the virulence and antibacterial susceptibility of E. coli strains isolated from patients with vaginitis can contribute to improved diagnosis and treatment of this disease.
Highlights
Vaginitis is one of the most common reasons women visit a gynecologist
One hundred and eighty cultures with E. coli were studied and separated into two groups: 90 isolates with single E. coli and 90 with E. coli associated to recognized aetiological agents of vaginitis such as Trichomonas vaginalis, C. albicans, Chlamydia trachomatis, Ureaplasma urealyticum, Mycoplasma hominis, Mobiluncus, Neisseria gonorroheae, and Streptococcus agalactiae and four strictly anaerobic bacterial species less frequently isolated from vaginitis such as Bacteroides fragilis, Fusobacterium nucleatum, Porphyromonas gingivalis and Prevotella intermedia [15]
Determination of virulence genes in E. coli strains obtained from vaginitis and its genetic combinations
Summary
Escherichia coli has been isolated from women with vaginitis, but its role as a vaginal infection aetiological agent is controversial. This study aimed to detect virulence genes and determine the antimicrobial susceptibility of E. coli strains isolated from monomicrobial and polymicrobial cultures collected from women with vaginitis. Results: A higher percentage (93.3%) of isolated strains from monomicrobial cultures with virulence genes in relation to polymicrobial cultures (56.7%) was found. Conclusions: Most of the E. coli strains isolated from monomicrobial cultures and some from polymicrobial cultures showed virulence genes. A better understanding of the virulence and antibacterial susceptibility of E. coli strains isolated from patients with vaginitis can contribute to improved diagnosis and treatment of this disease. By incorrectly assessing E. coli as a vaginal microbiota member, women infected with these bacterial species are not properly diagnosed or clinically treated
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