Abstract
Species of Staphylococcus are common in hospital infection (HI). Methicillin resistant S. aureus (MRSA) has also become a serious problem in Brazilian HI. The aim of this study was to characterize the pathogenicity of methicillin-resistant S. aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) isolated in public hospitals. The clinical isolates were obtained from intensive care unit. The MRSA and MSSA strains were genotyped by PCR for detection genes related to virulence factors. Moreover, the strains were tested for biofilm formation and cytokine induction in macrophages. Three strains of MRSA (9.68%) expressed the Sea gene, one (3.23%) Seb, 17 (54.84%) Spa and seven (22.58%) had PVL. Two MSSA strains (2.98%) expressed the Sea gene, three (4.48%) Seb, 18 (26.87%) Spa and 11 (16.42%) showed positive results for the PVL gene. There was no expression of Sec and CflA between MRSA and MSSA strains. Among MRSA and MSSA isolates, none statistical differences were observed in biofilm production. The analysis of cytokine induction in the inflammatory response of J774 macrophages by MRSA and MSSA isolates did not show statistical difference. Understanding the mechanisms of pathogenesis of S. aureus could provide important clues for both preventing and treating infection caused by these organisms.
Highlights
The Brazilian Ministry of Health defines Hospital Infection (HI) as that which is acquired during or after hospitalization, being possibly related to hospitalization or hospital procedures
The methicillin-resistant S. aureus (MRSA) and methicillin-sensitive Staphylococcus aureus (MSSA) samples were genotyped by Polymerase chain reaction (PCR) for detection of genes sea, seb (B), sec (C), PVL (Panton-Valentine Leukocidin), ClfA (Clumping Factor A) and spa
Some studies have found that Intensive Care Unit (ICU) patients are 5 to 10 times more likely to acquire an HI, and that this sector can accommodate approximately 20% of all hospital infections [14] [15]
Summary
The Brazilian Ministry of Health defines Hospital Infection (HI) as that which is acquired during or after hospitalization, being possibly related to hospitalization or hospital procedures. HI usually depends on the severity of patient disease at hospitalization, the nutritional status, diagnostic and therapeutic procedures, length of hospital stay, routine care, technical procedures and other features [2]. HI occurs mainly in patients with multiple invasive procedures that are in Intensive Care Unit (ICU), usually with a kind of imunosupression that requires antibiotic therapy [3]. Species of Staphylococcus are common in HI [4] and S. aureus methicillin resistant (MRSA) has become a serious problem in Brazilian HI. A total of 28% prevalence of S. aureus resistance to methicillin/oxacillin was found in HI at a Hospital in Salvador/BA. The highest detection was observed at patients in the ICU (59%), hemodialysis (43%), infectious diseases (34%) and neonatal units (18.5%) compared with the other local hospital [5]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
