Abstract
Background: Klebsiella pneumoniae (K. pneumoniae) significantly contributes to hospital-acquired bloodstream infections with high morbidity and mortality, especially biofilm-producing strains. It has been noticed that ability to produce biofilm by K. pneumoniae is linked to presence of some virulence genes, but this relationship needs further investigation. This work aimed to investigate ten virulence genes that may contribute to biofilm formation in K. pneumoniae strains causing bloodstream infections. Methods: This cross-sectional study included 108 K. pneumoniae isolates obtained from cases of hospital-acquired bloodstream Infections. The sensitivity to different antibiotics was tested by the disc diffusion method. Ability to form biofilm was detected by the tissue culture plate method. All isolates were tested by PCR to detect ten virulence genes suggested to be linked to biofilm formation ability. Results: The ability of biofilm formation was detected in 55.6% of the studied strains. Biofilm formation was more prevalent among wcaG, fimH, wabG, and mrkD positive isolates in comparison to negative isolates for the same genes. However, only wcaG and fimH genes have been found to be significantly associated with biofilm formation (P < 0.05). Conclusion: The association between the ability to form biofilm and the existence of wcaG and fimH genes in K. pneumoniae bacteremia isolates suggests these genes as promising therapeutic targets.
Highlights
Klebsiella pneumonia (K. pneumoniae) is aGram-negative rod that attracts worldwide attention due to marked increase in the rate of opportunistic and severe infections caused by the organism, as well as the growing antibiotic resistance with marked limitation in effective treatment options [1].Klebsiella pneumoniae significantly contributes to hospital-acquired bloodstream infections with a significant mortality rate (27–37%), especially in cases caused by multi-drug resistant (MDR) strains [2].Infections caused by biofilm-producing K. pneumoniae are more resistant to treatment than others
This study investigated K. pneumoniae strains causing hospital-acquired bloodstream infections isolated from a tertiary hospital in Egypt aiming to detect the genetically encoded virulence factors that may be linked to biofilm formation
K. pneumoniae isolates showed the ability of biofilm formation
Summary
Klebsiella pneumoniae significantly contributes to hospital-acquired bloodstream infections with a significant mortality rate Infections caused by biofilm-producing K. pneumoniae are more resistant to treatment than others. Biofilms act as shields for bacterial communities giving them more ability to escape the host defense mechanisms They prevent the access of different antibiotics giving the bacteria more resistance power. Klebsiella pneumoniae (K. pneumoniae) significantly contributes to hospital-acquired bloodstream infections with high morbidity and mortality, especially biofilm-producing strains. This work aimed to investigate ten virulence genes that may contribute to biofilm formation in K. pneumoniae strains causing bloodstream infections. All isolates were tested by polymerase chain reaction (PCR) to detect ten virulence genes suggested to be linked to biofilm formation ability. Conclusion: The association between the ability to form biofilm and the existence of wcaG and fimH genes in K. pneumoniae bacteremia isolates suggests these genes as promising therapeutic targets
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
