Abstract
Vibrio parahaemolyticus is the main pathogen of food-borne diarrheal in coastal areas. Through the study of pathogen characteristics of 1870 V. parahaemolyticus, the isolation rate of O4:KUT had increased significantly since 2013. In this study, we analyzed virulence, antimicrobial susceptibility, molecular, and epidemiological characteristics of a new serotype named O4:KUT2. O4:KUT2 strains had tlh+ tdh+ trh– toxRS/new– characteristics and were prevalent during 2013–2015. The 91.5% O4:KUT2 serotype strains were resistant to ampicillin. The growth curves of O4:KUT2 strains were different with O4:K9, O4:K8, and O3:K6 serotype strains. O4:KUT2 strains belonged to ST332 where four strains had a large fragment inserted at recA. Compared the whole genomes of O4:KUT2 strains with O4:K9 strain which also belonged to ST322 isolated from acute diarrhea patients in Zhejiang province in 2012, no different alleles at 2249 loci was found. This finding implied that O4:KUT2 strains might be derived from O4:K9 strains. Clinical presentation of patients positive for V. parahaemolyticus O4:KUT2 were no significant difference with patients positive for O3:K6, although their genetic characteristics were different. The appearance and the increase of proportion of the new serotype O4:KUT2 strains was aware that we should not relax the monitoring of the pathogen spectrum of acute diarrheal patients.
Highlights
Vibrio parahaemolyticus is the main pathogen of food-borne diseases in coastal areas (Klose and Satchell, 2018)
By BLAST K antigen gene clusters sequences of 16 whole genome sequenced O4:KUT2 serotype strains with 48 kinds of named K, we found that KUT2 antigen gene clusters sequences were specific and the highest identities to K9 and the second identities to K8
Based on the serotypes obtained by Polymerase chain reaction (PCR) screening and Whole-genome sequencing (WGS) were consistent in O4:KUT-recAin strains, we have not sequenced more O4:KUT2 strains
Summary
Vibrio parahaemolyticus is the main pathogen of food-borne diseases in coastal areas (Klose and Satchell, 2018). Ingestion of food containing V. parahaemolyticus can cause food poisoning. A New Serotype of Vibrio parahaemolyticus From Diarrhea Patients the outbreak of V. parahaemolyticus did not show obvious serotype specificity until February 1996, a large-scale food poisoning event broke out in Calcutta, India, caused by V. parahaemolyticus serotype O3:K6 (Okuda et al, 1997). The tlh+ tdh+ trh− toxRS/new+ genetic characteristics were found in at least 20 serotypes, including O3:K6, O4:K68, O4:K8, O1:K25, and O1:KUT (K untypeable) (Nair et al, 2007). The emergence of these pandemic characteristics was thought to be serotype transformation
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