Abstract

Entomopathogenic nematodes (EPNs) are key biocontrol agents against various insect pests such as pests of stored foods, when other control measures, such as pesticides are not appropriate. We evaluated the virulence and reproductive potential of four EPN isolates: Heterorhabditis bacteriophora MK263023, H. bacteriophora MK256358, H. bacteriophora MK256378 and S. cholashanense MH128155 against the larvae of the rice meal moth. These isolates were highly effective against the 4th instar larvae of the insect. All the isolates killed 100% of the insects within 24–48 h using 200 infective juveniles (IJs)/larvae. Similar mortality rates were observed at lower nematode concentrations, but more time was required. Heterorhabditis bacteriophora MK256358 and S. cholashanense MH128155 showed the best performance in killing and reproductive potential. Our results open new avenues for the potential of indigenous EPNs to effectively control this insect and set the basis for establishing safe strategy in its management programs in India.

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