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Abstract
As digital imaging technology advances, gigapixel or terapixel super resolution microscopic images become available. We have built a real time virtual microscopy technique for interactive analysis of super high resolution microscopic images over internet on standard desktops, laptops or mobile devices. The presented virtual microscopy technique is demonstrated to perform as fast as using a microscopy locally without any delay to assess gigapixel ultra high resolution image data through wired or wireless internet by a Tablet or a standard PC. More importantly, the presented technology enables analysis of super high resolution microscopic image across sites and time and allows multi-person analysis at the same time, which greatly speed up data analysis process and reduces miscommunication among scientists and doctors. A web site has been created for illustration purposes. (http://www-o.ntust.edu.tw/~cweiwang/VirtualMicroscopy).
Highlights
In this work, we have built a real time virtual microscopy technique for interactive analysis of super high resolution microscopic images over internet
The presented technology enables analysis of super high resolution microscopic image across regions and time and allows multi-person analysis at the same time, which greatly speed up data analysis process and reduces miscommunication among scientists and doctors
As it is impossible to obtain the same images to test in each benchmark system, images in the benchmark systems with the closest dimension were chosen to test, and the size of the super resolution images in the individual benchmark systems is 3.7 GB and 12.78 GB (Leica Aperio WebScope), respectively
Summary
We have built a real time virtual microscopy technique for interactive analysis of super high resolution microscopic images over internet. The presented virtual microscopy technique is demonstrated to perform as fast as using a microscopy locally without any delay. The presented technology enables analysis of super high resolution microscopic image across regions and time and allows multi-person analysis at the same time, which greatly speed up data analysis process and reduces miscommunication among scientists and doctors. In comparison with the state of the art methods, including Microsoft HDview[3] and Leica Aperio WebScope[4], the presented technique is significantly faster (p < 0 .001 according to Tukey’s HSD and LSD tests). A virtual microscopy demonstration on a standard PC through wired internet is given in the supplementary video 3, and a demonstration on a Tablet through wireless internet is given in the supplementary video 4
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