Abstract
The molecular weight and isoelectric point (pI) of the proteins plays important role in the cell. Depending upon the shape, size, and charge, protein provides its functional role in different parts of the cell. Therefore, understanding to the knowledge of their molecular weight and charges is (pI) is very important. Therefore, we conducted a proteome-wide analysis of protein sequences of 689 fungal species (7.15 million protein sequences) and construct a virtual 2-D map of the fungal proteome. The analysis of the constructed map revealed the presence of a bimodal distribution of fungal proteomes. The molecular mass of individual fungal proteins ranged from 0.202 to 2546.166 kDa and the predicted isoelectric point (pI) ranged from 1.85 to 13.759 while average molecular weight of fungal proteome was 50.98 kDa. A non-ribosomal peptide synthase (RFU80400.1) found in Trichoderma arundinaceum was identified as the largest protein in the fungal kingdom. The collective fungal proteome is dominated by the presence of acidic rather than basic pI proteins and Leu is the most abundant amino acid while Cys is the least abundant amino acid. Aspergillus ustus encodes the highest percentage (76.62%) of acidic pI proteins while Nosema ceranae was found to encode the highest percentage (66.15%) of basic pI proteins. Selenocysteine and pyrrolysine amino acids were not found in any of the analysed fungal proteomes. Although the molecular weight and pI of the protein are of enormous important to understand their functional roles, the amino acid compositions of the fungal protein will enable us to understand the synonymous codon usage in the fungal kingdom. The small peptides identified during the study can provide additional biotechnological implication.
Highlights
The molecular weight and isoelectric point of the proteins plays important role in the cell
The largest genome size in the fungal kingdom was found in Neocallimastix californiae (193.032 Mb) followed by Tuber magnatum (192.781 Mb) (Supplementary File 2)
When we searched for Sec-encoding protein sequences in the 7.15 million fungal proteome sequences from 689 species, we found that 134 sequences in 112 fungal species had amino acid sequences with an annotation name “selenocysteine” (Supplementary File 4)
Summary
The molecular weight and isoelectric point (pI) of the proteins plays important role in the cell. Proteins play a qualitative and quantitative role in growth, development, and stress tolerance of an o rganism[3,4,5,6] They are the structural and functional units of a cell comprising a chain of amino acids which are arranged as determined by their coding sequences (mRNA)[7,8,9]. The sub-cellular localization of a protein plays a critical role in defining its function since different cellular compartments regulate various physiological and biochemical functions[18,19,20]. The annotated protein sequences of 689 species derived from completed genome sequencing efforts within public repositories were used to construct a collective 2-D map of the fungal proteome
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