Abstract
BackgroundThe mosquito Aedes albopictus is an important vector for many pathogens. Understanding the virome in Ae. albopictus is critical for assessing the risk of disease transmission, implementation of vector control measures, and health system strengthening.MethodsIn this study, viral metagenomic and PCR methods were used to reveal the virome in adult Ae. albopictus captured in different areas and during different seasons in Guangzhou, China.ResultsThe viral composition of adult Ae. albopictus varied mainly between seasons. Over 50 viral families were found, which were specific to vertebrates, invertebrates, plants, fungi, bacteria, and protozoa. In rural areas, Siphoviridae (6.5%) was the most common viral family harbored by mosquitoes captured during winter and spring, while Luteoviridae (1.1%) was the most common viral family harbored by mosquitoes captured during summer and autumn. Myoviridae (7.0% and 1.3%) was the most common viral family in mosquitoes captured in urban areas during all seasons. Hepatitis B virus (HBV) was detected by PCR in a female mosquito pool. The first near full-length HBV genome from Ae. albopictus was amplified, which showed a high level of similarity with human HBV genotype B sequences. Human parechovirus (HPeV) was detected in male and female mosquito pools, and the sequences were clustered with HPeV 1 and 3 sequences.ConclusionsLarge numbers of viral species were found in adult Ae. albopictus, including viruses from vertebrates, insects, and plants. The viral composition in Ae. albopictus mainly varied between seasons. Herein, we are the first to report the detection of HPeV and HBV in mosquitoes. This study not only provides valuable information for the control and prevention of mosquito-borne diseases, but it also demonstrates the feasibility of xenosurveillance.Graphical
Highlights
The mosquito Aedes albopictus is an important vector for many pathogens
Viral metagenomic and polymerase chain reaction (PCR) methods were used to reveal the viral composition in adult Ae. albopictus captured in different areas and during different seasons in Guangzhou City, China
Viral metagenomics was performed to investigate the virome in adult Ae. albopictus captured in different areas and during different seasons in Guangzhou City, Guangdong Province, China
Summary
The mosquito Aedes albopictus is an important vector for many pathogens. Understanding the virome in Ae. albopictus is critical for assessing the risk of disease transmission, implementation of vector control measures, and health system strengthening. Ae. albopictus is a competent vector of at least 22 arboviruses that can cause human infections, including Zika. He et al Parasites Vectors (2021) 14:415 virus (ZIKV), dengue virus (DENV), chikungunya virus (CHIKV), and yellow fever virus (YFV) [5]. Outbreaks caused by arboviruses have been reported, such as dengue fever in Thailand, Vietnam, and China, and chikungunya in France, Tanzania, Africa, and the Americas [2, 6,7,8,9,10,11,12,13]. China is a country located in East Asia. Ae. albopictus is found in nearly one third of the regions in China: south to Hainan Island, north to Shenyang and Dalian, west to Jingshui and Longnan, southwest to the Tibet Autonomous Region, and most regions to the east [18,19,20]
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