Abstract
In recent years, African swine fever (ASF) has been the main threat to the development of the pig industry not only in Russia, but also in many countries of the world for many years. Due to the lack of effective and safe means of specific prevention of infection, disinfection is one of the ways to combat and prevent the spread of the ASF pathogen in the system of antiepizootic measures. The most important task of modern disinfection is the development and creation of disinfectants with a wide range of bactericidal and viricidal effects. The composition of such despreprates includes several active substances that, due to interaction with each other, enhance the disinfecting ability of the drug.The aim of the work is to study in vitro the viricidal activity of the composite disinfectants "Dezon Vetklin" and "Dezon Vet" against a highly virulent ASF pathogen. The objects of research are domestic modern composite disinfectants "Dezon Vetklin" and "Dezon Vet". The tests of the drugs were carried out on the basis of the Federal State Budgetary Institution "Federal Center for Animal Health Protection" (FGBI "VNIIZH", Vladimir). As a test culture, a primary culture of pig spleen cells sensitive to ASF virus (CCSS) was used with the addition of Igla growth culture medium containing 20% fetal serum of cattle and 0.1% (v/v) pig erythrocytes. As a protein load, inactivated pig blood serum obtained from animals from ASF-safe farms of the Russian Federation was used. For experimental infection of CCSS, a highly virulent hemadsorbent ASF virus of genotype II strain ASF/Arm/07 (reference) was used in a titer not lower than 6.0 lg GAdE50/cm3. For experimental testing of disinfectants, metal, wooden, concrete and plastic test surfaces with a size of 10x10 cm were used. According to the results of the studies, viricidal activity against the ASF pathogen of two tested disinfectants was established at a concentration of 5.0% with a minimum exposure of 10 minutes each. The tested disinfectants can be recommended for preventive disinfection at veterinary surveillance facilities in ASF.
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