Virgibacillus campisalis sp. nov., from a marine solar saltern

Abstract

A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20(T), was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20(T) grew optimally at 37 °C, at pH 7.5-8.0 and in the presence of 4-5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20(T) belongs to the genus Virgibacillus. Strain IDS-20(T) exhibited 93.4-96.6 % 16S rRNA gene sequence similarity to the type strains of species of the genus Virgibacillus. Strain IDS-20(T) had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on meso-diaminopimelic acid. The major fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20(T) demonstrated that this strain can be distinguished from recognized species of the genus Virgibacillus. On the basis of the data presented, strain IDS-20(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus campisalis sp. nov. is proposed. The type strain is IDS-20(T) ( = KCTC 13727(T) = CCUG 59308(T)).