Virally mediated functional suppression of GABA-A receptors in the rodent thalamus

Abstract

Event Abstract Back to Event Virally mediated functional suppression of GABA-A receptors in the rodent thalamus Z. Rovó1*, F. Mátyás1, P. Barthó1, A. Slézia1 and L. Acsády1 1 Hungarian Academy of Sciences, Institute of Experimental Medicine, Hungary GABAergic inhibition is essential for the normal function of thalamocortical networks. In addition beside the phasic inhibition mediated by synaptic GABA-A receptors, extrasynaptic GABA-A receptors also exert powerful tonic inhibition on thalamocortical cells. The nucleus specific role of synaptic inhibition is difficult to assess using the traditional pharmacological or KO methods due to the limited spatial and temporal control of their effects. Thus, in this study, we used stereotaxic injection of viral particles to focally knock down synaptic GABA-A receptors. All synaptic GABA-A receptors in the thalamus contain g2 subunit. Injection of AAV-Cre viral particles into the thalamus of g2 floxed mice results in the focal removal of the g2 gene. Successful removal of the g2 gene was identified by immunocytochemistry for g2 protein. Immunoreactivity of the other subunits of synaptic GABA-A receptors (a1, b2) as well as extrasynaptic receptors (alpha4) remained unchanged. Preliminary juxtacellular recording in anaesthetized mice demonstrated that it is possible to record, label and identify virally infected thalamocortical cells, which lack functional synaptic GABA-A receptors and to differentiate them from non-infected cells with intact inhibition. Our data demonstrate that the present method is an excellent tool to study the nucleus specific role of synaptic GABA-A receptor in cellular and network activity. Keywords: Neurophysiology, Neuroscience Conference: 13th Conference of the Hungarian Neuroscience Society (MITT), Budapest, Hungary, 20 Jan - 22 Jan, 2011. Presentation Type: Abstract Topic: Neurophysiology Citation: Rovó Z, Mátyás F, Barthó P, Slézia A and Acsády L (2011). Virally mediated functional suppression of GABA-A receptors in the rodent thalamus. Front. Neurosci. Conference Abstract: 13th Conference of the Hungarian Neuroscience Society (MITT). doi: 10.3389/conf.fnins.2011.84.00026 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 03 Mar 2011; Published Online: 23 Mar 2011. * Correspondence: Dr. Z. Rovó, Hungarian Academy of Sciences, Institute of Experimental Medicine, Budapest, Hungary, rovozita@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Google Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Google Scholar Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády PubMed Z. Rovó F. Mátyás P. Barthó A. Slézia L. Acsády Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.