Abstract

The development of virus protein in Vicia faba plants following inoculation with broadbean mottle virus (BBMV) was studied by means of immunoradioautography. Antigen was found in the nucleus and the cytoplasm on the first day of infection. It then accumulated rapidly in the cytoplasm as infection progressed, and high concentrations of antigen were present in the cytoplasm by the fourth day after infection. Chloroplasts were not labeled. Viral antigen was always associated with viral inclusion bodies. The results with BBMV antigen are compared with those in earlier reports on the appearance of tobacco mosaic virus and clover yellow mosaic virus antigen.

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