Abstract
A poliovirus-specific protein involved in viral RNA synthesis in vitro has been previously identified and partially purified. A second viral protein which purifies with the replication complex is described in this paper. A comparison of tryptic peptide maps of noncapsid viral protein 2 with the two replication complex-associated proteins indicates that all three are related. We designate this newly identified protein as 4a and refer to the previously identified protein as 4b. The apparent molecular weights of 4a and 4b by SDS-PAGE are 65,000 and 58,000, respectively. Pulse-chase experiments suggest that 4a may be a precursor to 4b. It is degraded within about an hour after being synthesized, and its degradation is inhibited by treatment of infected cells with iodoacetamide. An unstable precursor with the properties of 4a could conceivably contain the sequence for the 5′ terminally linked protein, VPg.
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