Viral community in landfill leachate: Occurrence, bacterial hosts, mediation antibiotic resistance gene dissemination, and function in municipal solid waste decomposition

Abstract

A municipal solid waste (MSW) landfill is a significant source of antibiotic resistance, pathogens and viruses and also a habitat for microbial consortia that perform MSW decomposition. Viruses are of great significance in ecological interactions such as MSW decomposition and antibiotic resistance gene (ARG) transmission. In this study, the viral community structure and the associated driver, the linkage of viruses and their bacterial hosts, the virus-associated ARG dissemination and virtual community function on MSW decomposition were investigated in landfill leachate from seven cities, China. The seven cities include four megacities, two large-scale cities and one small-scale city, representing the leachate characters of China. The results showed that the leachates were dominated by the phage families Siphoviridae, Myoviridae and Podoviridae (91.7 ± 3.6) %. Their putative hosts were the important MSW decomposers Lactobacillus, Pseudomonas, Clostridium, Proteiniphilum, and Bacteroides. The structure of the viral community was significantly affected by pH (P = 0.007, analyzed by RDA) and the bacterial community (R = 0.83, P < 0.001, analyzed by Mantel test). The relative abundance of ARGs showed a strong correlation (R > 0.8, P < 0.01) with viral family, suggesting that viruses play an important role in ARGs dissemination. Phage regulate bacterial population abundance through top-down effects, thus participating in MSW decomposition. These results demonstrate that viral community are involve in ARGs transmission and dissemination and mediate MSW decomposition in landfill.