Abstract
The aim of study was to assess whether vinpocetine (VINP) has protective effect on astrocytes in ischemic injury. Primary cultures of rat astrocytes were deprived of glucose and serum and incubated for 24 h in 3% O2 with 0.1 and 10 μm of VINP either during 24 h normoxia, 24 h simulated ischemia or 24 h reoxygenation. Cells viability was determined by Live/Dead Assay Kit, LDH release and MTT [3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide] conversion tests. We measured ATP concentration by bioluminescent method and apoptosis by staining with Hoechst 33342 and caspase‐3 assay. Treatment with 0.1 μm VINP during ischemia and reoxygenation significantly decreased the number of apoptotic cells as well as caspase‐3 activity, and increased ATP level and mitochondrial function. Higher VINP concentration (10 μm) was detrimental during ischemia. Experiments proved cytoprotective effect of 0.1 μm VINP on astrocytes in vitro.
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