Abstract
Abstract: The aim of this study was to adapt the vigor test methodology of (strong) normal intact seedlings of Amburana cearensis and evaluate efficiency in physiological classification of seed lots. The study was conducted in two stages: morphological characterization of seedlings and physiological analysis of seed lots. To do so, the following tests were carried out: (strong) normal seedlings, germination, emergence, first count, germination speed index, tetrazolium, length of strong normal seedlings, length and dry mass of seedlings. The experimental design was completely randomized. The Tukey and Kruskal-Wallis tests were used to compare the results, and the correlation between the variables was analyzed by Spearman and Pearson coefficients. The seedlings of A. cearensis are semi-hypogeal phanerocotylar, with development of normal seedlings on the ninth day after sowing. The vigor test of strong normal seedlings, length of strong normal seedlings, dry matter, and tetrazolium led to physiological classification into different vigor levels. The vigor test of (strong) normal seedlings, the length of strong normal seedlings, and tetrazolium (vigor) were effective for vigor classification of A. cearensis seeds.
Highlights
A plant native to the Caatinga ecosystem, Amburana cearensis (Fabaceae) is known as cumaru or amburana-de-cheiro (Almeida et al, 2017)
A. cearensis has been used for food purposes and in creation of soaps and perfumes, but it is the object of pharmacological studies, which have confirmed the effectiveness of bark extracts as antiseptics against bacteria (Sá et al, 2014) and for anti-inflammatory activity (Lopes et al, 2013)
Seedling vigor tests are promising for evaluating the physiological quality of seed lots, for they are able to predict the development and establishment of seedlings in the field
Summary
A plant native to the Caatinga (xeric shrubland) ecosystem, Amburana cearensis (Fabaceae) is known as cumaru or amburana-de-cheiro (Almeida et al, 2017). Intact normal seedlings can be defined as those that have developed, complete, and healthy essential structures (Brasil, 2013), and as such can be considered “strong normal seedlings”, whereas seedlings with small defects and/or secondary infection can be classified as “weak normal seedlings” (Krzyzanowski and Nakagawa, 1999). These authors affirm that seed lots that have a higher percentage of intact normal seedlings have greater chances of emerging and producing normal plants under adverse field conditions
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