Abstract
Seed marks the beginning of grain production from cultivated crops. Ensuring seed quality is the priority in the field of seed technology. In this context, the purpose of this study was to evaluate the use of carbon dioxide concentration to Classify Brassica napus seed lots of different quality resulting from various sowing dates. Seed lots were evaluated by percentage of normal plantlets and the germination rate index. CO2 concentration was quantified in samples submitted to five periods of incubation at 40° C with the aid of a gas exchange analyzer coupled to an injection and airflow system. The results were subjected to analysis of variance and the mean values were compared by the Duncan test and by regression analysis at the 5% level of significance. B. napus seeds from all lots showed a high percentage of normal plantlets and germination rate index. Percentage of normal plantlets was correlated with CO2 concentration. Determination of the CO2 concentration was more efficient with three hours of incubation which allowed classification of canola seed lots into four vigor classes. CO2 concentration was inversely proportional to the percentage of normal plantlets.
Highlights
Seed quality results from the aggregate of genetic, physical, physiological, biochemical, agro-climatic and seed health components that affect the capacity of producing plants with greater yield (MARCOS-FILHO, 2005)
The purpose of the present study was to evaluate the use of carbon dioxide concentration with an infrared gas analyser in classification of Brassica napus L. seeds of different levels of quality
The germination tests was conducted with four replications of 50 seeds per lot in blotting paper substrate, kept in Gerbox® boxes maintained in BOD at 20.0 ± 2.0o C with the results expressed in percentage of seeds producing normal plantlets seven days after sowing (BRASIL, 2009)
Summary
Seed quality results from the aggregate of genetic, physical, physiological, biochemical, agro-climatic and seed health components that affect the capacity of producing plants with greater yield (MARCOS-FILHO, 2005). The greatest interest in quantifying the physiological quality of seeds is bound up with the importance of obtaining rapid and reliable results that aid in decision making at different stages of seed production and storage. With the beginning of hydration, gene transcription and translation are resumed in orthodox seeds, culminating in catabolic activation of enzymes. All these physiological processes require consumption of energy generated by aerobic as well as anaerobic respiration.
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