Vigor and oxidation reactions in soybean seedlings submitted to different seed chemical treatments

Maíra Paes Lacerda,Renan Caldas Umburanas,Durval Dourado-Neto,Marco Antonio Tavares Rodrigues,Karla Vilaça Martins,Klaus Reichardt

doi:10.1590/2317-1545v43247033

Maíra Paes Lacerda, Renan Caldas Umburanas + Show 4 more

Open Access

https://doi.org/10.1590/2317-1545v43247033

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Abstract

Abstract: The interaction of different seed chemical treatment with seedlings germination, vigor and stress metabolism attributes needs to be better understood in soybean crop. The objectives of this study were to evaluate seed vigor, germination, and biochemical attributes of soybean seedlings as a function of different seed chemical treatments. A completely randomized experimental design was adopted, with 8 seed treatments and 4 replicates. Seed germination, vigor (first germination count, cold test, accelerated aging, emergence speed index), seedling biomass, enzimes activity and SVIS® analysis were evaluated. The evaluated treatments had no difference relative to control on seed germination and vigor. Through image analysis, the seedling uniformity index were equal or higher than control with the seed chemical treatment, while vigor and growth indexes had no difference. Shoot biomass of seedlings tends to decrease with seed chemical treatments, and root biomass had no difference. Seed chemical treatments increased the activity of hydrogen peroxide in seedlings by 6 to 40% compared to control; the activity of catalase increased by 87 to 184% compared to control; and no effect was observed in lipids peroxidation. Overall, seed chemical treatment benefited the physiological activity of soybean seedlings and improved antioxidant metabolism activity. None of the seed chemical treatments used were phytotoxic.

Highlights

During the germination process seedlings are subject to biotic and abiotic stresses
Cold test showed that the germination of seeds ranged from 87 to 95% and was higher for the Fipronil treatment compared to Fipronil + Pyraclostrobin + Thiophanate-methyl treatment, while the other treatments did not differ among themselves (Table 1)
The evaluated seed chemical treatments have no difference relative to control on seed germination and vigor by the germination test on paper, cold test, accelerated aging, and emergence speed index

Summary

Introduction

During the germination process seedlings are subject to biotic and abiotic stresses. These can reduce the vigor of the plant, delay plant development or even result in death of the seed and/or seedling in the early stages of germination. Chemical treatment of seeds with pesticides is an effective method to reduce biotic stresses such as soil diseases, but there are still limited information of how such treatments affect seed vigor and, specially, attributes of stress metabolism. Such information could lead to a better understanding of how these compounds affect seed viability and seedling development, as well improve its use. The seed physiological potential can be evaluated by germination tests, seeds with good physiological potential do not always present a good emergence, uniformity, and adequate plant density in the field, because stress factors, such as the presence of soil pests and pathogens, can impair this potential, especially in tropical environments

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