Abstract
Carey suggested that chick-embryo ribosome tetramers attach to the substrate film preferentially in one of the two possible orientations, making them particularly appropriate for use in the three-dimensional reconstruction of ribosome structure. We have stored and analyzed tetramer images digitally as described earlier. To test for a fixed orientation, many tetramer Images were compared to a reference image that contained an arbitrarily labelled "right-handed" view. Images that correlated well with the reference were averaged, giving a right-handed result (fig. 1). To search for a left-handed view, the reference image was "turned over” in the computer to artificially create a left-handed image. Repeating the procedure with the new reference gave a result that was mostly symmetric (fig. 2), not left-handed.
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