Abstract
Here I comment on the articles by Lee and colleagues (Retrovirology 2011, 8:82) and Lee and Cho (Retrovirology 2012, 9:23) dealing with an endogenous ecotropic mouse leukemia virus found in C57BL mice.
Highlights
I comment on the articles by Lee and colleagues (Retrovirology 2011, 8:82) and Lee and Cho (Retrovirology 2012, 9:23) dealing with an endogenous ecotropic mouse leukemia virus found in C57BL mice
It is apparent that some confusion has resulted from the fact that the sequenced mouse genome is poorly annotated for retroviral sequences, from observations that the Emv2 provirus is replication incompetent, and because the original map location of Emv2 on the Chromosome (Chr) 8 linkage map is not precisely identical to its location on the database map Build
As pointed out by Young and colleagues [3], Southern blot analysis of C57BL DNA and examination of its sequenced genome identify one and only one ecotropic MLV provirus in this mouse strain that is found in the BAC RP23-214K5 (GenBank No AC158362, position 97057-105700); this full-length provirus, Emv2, is 99.5% identical to the infectious AKV MLV derived from Emv11 (J01998)
Summary
I comment on the articles by Lee and colleagues (Retrovirology 2011, 8:82) and Lee and Cho (Retrovirology 2012, 9:23) dealing with an endogenous ecotropic mouse leukemia virus found in C57BL mice. It is worthwhile elaborating on several points relevant to this issue: the fact that mice carrying only Emv2 can, produce infectious virus, and the evidence supporting the map location of this functional E-MLV provirus on distal Chromosome (Chr) 8 of C57BL mice. As pointed out by Young and colleagues [3], Southern blot analysis of C57BL DNA and examination of its sequenced genome identify one and only one ecotropic MLV provirus in this mouse strain that is found in the BAC RP23-214K5 (GenBank No AC158362, position 97057-105700); this full-length provirus, Emv2, is 99.5% identical to the infectious AKV MLV derived from Emv11 (J01998).
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