Abstract
Videomicroscopy is the appropriate method to study many morphological and intracellular changes of individual cells. In this chapter the two principal types of videomicroscopy will be described: videomicroscopy with normal phase contrast, and video enhanced contrast differential interference contrast (VECDIC) microscopy. The first is especially suited to study morphological changes of the cell such as cell movement, retraction, outgrowth, filopodia dynamics (Nobes and Hall, 1995), and mitochondria movement. VECDIC, in contrast, is based on Normarski microscopy. With this method it is possible to track moving vesicles in neurites (Allen et al. 1982; see also Fig. 4.1) and lamellipodia (Fisher et al. 1988), microtubule polymerization (Cassimeris et al. 1988), and actin dynamics in growth cones (Forscher and Smith 1988). Beside these methods, organelle transport can be also specifically visualized and documented by fluorescent microscopy using specific fluorescent probes (Lee and Chen 1988).
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