Abstract
Phage N4 is one of the five newly isolated phages used in a new phage typing scheme for Vibrio cholerae O1 biovar EITor strains and belongs to the Podoviridae family. Electron microscopic studies showed that the phage N4 has a DNA of 40.4 ± 0.1 kb. A partial denaturation map of the N4 DNA has been constructed. It has been shown with the help of this partial denaturation map that phage N4 genome is nonpermuted. Circularization of the phage genome on treatment with exonuclease III, followed by incubation in 50% formamide, indicates a terminal redundancy.Key words: vibriophage N4, DNA, Vibrio cholerae, denaturation mapping.
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