Abstract
A novel Vibrio strain, JCM 31412T, was isolated from seawater collected from the Inland Sea (Setonaikai), Japan, and characterized as a Gram-negative, oxidase-positive, catalase-negative, facultatively anaerobic, motile, ovoid-shaped bacterium with one polar flagellum. Based on 16S rDNA gene identity, strain JCM 31412T showed a close relationship with type strains of Vibrio brasiliensis (LMG 20546T, 98.2% identity), V. harveyi (NBRC 15634T, 98.2%), V. caribbeanicus (ATCC BAA-2122T, 97.8%) and V. proteolyticus (NBRC 13287T, 97.8%). The G+C content of strain JCM 31412T DNA was 46.8%. Multi-locus sequence analysis (MLSA) of eight loci (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA; 5535bp) further clustered strain JCM 31412T in the Nereis clade, genus Vibrio. Phenotypically, strain JCM 31412T differed from the closest related Vibrio species in its utilization of melibiose and raffinose, and its lack of casein and gelatin hydrolysis. It was further differentiated based on its fatty acid composition, specifically properties of C12:03OH and summed features, which were significantly different from those of V. brasiliensis, V. nigripulchritudo and V. caribbeanicus type strains. Overall, the results of DNA-DNA hybridization, and physiological and biochemical analysis differentiated strain JCM 31412T from other described species of the genus Vibrio. Based on these polyphasic taxonomic findings, it was therefore concluded that JCM 31412T was a novel Vibrio species, for which the name Vibrio japonicus sp. nov. was proposed, with JCM 31412T (= LMG 29636T = ATCC TSD-62T) as the type strain.
Highlights
The family Vibrionaceae, in class Gammaproteobacteria, currently includes nine recognized genera: Vibrio [1], Photobacterium [2], Catenococcus [3], Grimontia [4], Echinimonas [5], Salinivibrio [6], Enterovibrio [7] and Aliivibrio [8]
We describe a new species isolated from seawater from the Inland Sea, Japan, for which the name Vibrio japonicus is proposed
Strain JCM 31412T was deposited in the Japan Collection of Microorganisms (JCM), Microbe Division, RIKEN BioResource Center, Ibaraki, Japan, the BCCM/LMG Bacteria Collection at Ghent University (Belgian Coordinated Collection of Microorganisms), Gent, Belgium and the American Type Culture Collection (ATCC), Manassas, VA, USA
Summary
The family Vibrionaceae, in class Gammaproteobacteria, currently includes nine recognized genera: Vibrio [1], Photobacterium [2], Catenococcus [3], Grimontia [4], Echinimonas [5], Salinivibrio [6], Enterovibrio [7] and Aliivibrio [8]. 131 species of Vibrio including two subspecies have been described (www.bacterio.net/vibrio/html). Vibrio species are halophilic, mesophilic or chemoorganotrophic in nature, with facultatively fermentative metabolism [11]. They are ubiquitous inhabitants of aquatic environments including marine, coastal, freshwater and sediments, as well as being eukaryotic hosts [12,13,14]. We describe a new species (strain JCM 31412T) isolated from seawater from the Inland Sea, Japan, for which the name Vibrio japonicus is proposed
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