Abstract

This study aims to investigate the effects of inulin and fructooligosaccharides (FOS) supplementation on the viability, storage stability, and in vitro gastrointestinal tolerance of Lactiplantibacillus plantarum in different sugar systems using 24 h growth and 10 days survival studies at 37 °C, inulin, and FOS (0%, 0.5%, 1%, 2%, 3% and 4%) supplementation in 2%, 3%, and 4% glucose, fructose, lactose, and sucrose systems. Based on the highest percentage increase in growth index, sucrose and lactose were more suitable sugar substrates for inulin and FOS supplementation. In survival studies, based on cell viability, inulin supplementation showed a better protective effect than FOS in 3% and 4% sucrose and lactose systems. Four selected sucrose and lactose systems supplemented with inulin and FOS were used in a 12-week storage stability study at 4 °C. Inulin (3%, 4%) and FOS (2%, 4%) supplementation in sucrose and lactose systems greatly enhanced the refrigerated storage stability of L. plantarum. In the gastrointestinal tolerance study, an increase in the bacterial survival rate (%) showed that the supplementation of FOS in lactose and sucrose systems improved the storage viability of L. plantarum. Both inulin and FOS supplementation in sucrose and lactose systems improved the hydrophobicity, auto-aggregation, co-aggregation ability of L. plantarum with Escherichia coli and Enterococcus faecalis.

Highlights

  • 75% stands for growth kinetics similar to those under optimal conditions; growth index in the range of 25–75% underlines a partial inhibition; growth index < 25% stands for potent inhibition of the microorganism [38]

  • The growth index of L. plantarum increased with increasing inulin and FOS concentration from 0.5% to 3% as a sole carbon source in MRS

  • In growth assay and survival studies, the highest percentage increase in growth index and cell viability showed that sucrose and lactose were more suitable sugar substrates for inulin and FOS supplementation

Read more

Summary

Introduction

Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. Inulin and fructooligosaccharides (FOS) are among the most studied and well-established prebiotics. Inulin and FOS consist of a linear chain of fructose, constituted by a monomeric unit of fructose linked by beta glycosidic (2, 1) bonds, with a terminal glucose unit. Inulin has a heterogeneous degree of polymerization ranging (DP) from 6 to 60, while FOS has a DP ranging from 2 to 10 [1,2,3]. The inability of the human digestive system to hydrolyze fructans is due to the lack of effective hydrolytic enzymes that can break β linkages [4]

Objectives
Methods
Results
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call