Abstract
The cell viability and DNA damage in unstimulated sheep primary lymphocytes subjected to different extremely low electromagnetic field intensities (5, 50 and 100 µT; 50 Hz) were studied with special emphasis on apoptosis. Sheep primary lymphocytes cultured in RPMI, supplemented with 10% FBS in the absence of mitogens, were exposed till 16 h. The cell viability assessment by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide (MTT) assay showed a dose dependent enhancement of viability at 16 h. Further, quantitative DNA laddering and flow cytometric analysis showed a significant decrease in apoptosis of the cells subjected to 100 (p
Highlights
The biological effects caused by Extremely Low Frequency Electromagnetic fields (ELF-EMF) have been undergoing serious debate since the last two decades
Significant (p
Peripheral lymphocytes have been the cellular model of choice because they are well characterized cells as far as culture conditions and growth factor requirements are concerned
Summary
The biological effects caused by Extremely Low Frequency Electromagnetic fields (ELF-EMF) have been undergoing serious debate since the last two decades. The available biological data on the oncogenic potential of ELF-EMF are unable to provide a cause-effect relationship between exposure conditions and induction of tumors In this context, the question of possible genotoxic effects is relevant. The latest published data provided evidence suggesting that ELF-EMF interfere with the DNA repair process leading to accumulation of damaged DNA in cells [13] It was stated by Philip et al, 1996 [11], that exposure of Molt 4 T- lymphoblastoid cells to a 100 μT, 60 Hz sinusoidal magnetic fields leads to decreased activity of the DNA repair enzyme, poly (ADP ribose) polymerase, and increased DNA fragmentation in the presence of the DNA damaging agent, etoposide. Environmental level magnetic fields (10, 50 and 100 μT) had no effect on in vitro transcription in a cell free system [18]
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