Viability of simultaneous analysis through reagent mixture in microfluidic paper-based analytical devices using colorimetric measures

Abstract

In this project, the viability and adequacy of simultaneous analysis conducted in a single reactional zone of Microfluidic Paper-based Analytical Device (?PAD) though the mixure of 2 or more colorimetric reagents was evaluated for synthetic samples with the concentration range of 0 to 100 ppm for the analytes. The analytical system used in this research was based on a combination of the aluminum chloride method to quantify flavonoids through complex formation and the Folin-Ciocalteu reagent method to quantify the total phenolic content through redox reactions. Both methods were adapted from their original spectrophotometric systems to new microfluidic ones.