Abstract
Viability of Rhizobium leguminosarum bacteroids isolated from Pisum sativum was determined after sucrose gradient centrifugation of the bacteroids resulting in separation according to developmental stage. The results suggest that past a critical stage of development bacteroids lose viability. Similar experiments with R. japonicum bacteroids having a lower degree of morphological differentiation showed an appreciably higher viability in all gradient fractions. The results support the hypothesis that bacteroid morphology and viability is dependent on the nature of—and length of exposure to—the host plant cytoplasm.
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