Viability of ram’s X-Y sperm after sexing with bovine serume albumin at different incubation time

Abstract

Sperm sexing technology was done to increase reproductive efficiency due to artificial insemination (AI) programe. It can improve the proportion of certain chromosome bearing sperm from its natural proportion, but there is not information about the viability of sexed sperm with Bovine Serume Albumin (BSA) in Local Ram. The aims of the research were to find out the viability of X-Y sperm after sexing with BSA at different incubation time, which one of the incubation time that maintain the longest viability and to find out which kind of sperm that have higher viability. This research was done with completely randomized design with three treatments of incubation time (45, 60, and 75 minute) and eight replications. The sexing method was used is colums of BSA. Parameters that were evaluated were viability of X-sperm and Y-sperm up to 40% and 0% motility. Result showed that incubation time significantly effect on X and Y-sperm at 0% motility. X-sperm viability at incubation time 45, 60 and 75 minute were 5.5, 5.0 and 4.33 days resfectively at 40%motility, and about 12.38, 11.88 and 11.50 days at 0% motility. Viability of Y-sperm at incubation time for 45, 60 and 75 minute were 4.17, 4.0 and 3.5 days up to 40% motility and 10.75, 10.13 and 9.5 days up to 0% motility. It is concluded that the viability of X and Y-sperm were resulted from 45 minute of incubation time longer than 60 and 75 minute incubation time both of up to 40% and 0% motility, and the X-sperm have highest viability than Y sperm.