Abstract
Survival of Pisolithus tinctorius grown in liquid medium and subsequently encased in beads of calcium alginate was tested under different conditions. Mycelium viability was not affected by the addition of peat or bentonite to the alginate gel. Both peat and bentonite improved the water retention of the alginate gel, but addition of only bentonite slowed down the rate of moisture loss by evaporation. At 4 C the entrapped mycelium retained its viability for 5 mo. Both sterile and nonsterile nursery soil supported growth of hyphae from beads containing peat or bentonite or only alginate. On nonsterile soil, hyphae were not abundant and those that developed began to die off sooner than those on sterile soil. The objective of the present study is to know the effect of peat or bentonite on the survival of Pisolithus tinctorius encased in alginate gel for production of effective and disseminate inoculum package for use in commercial nurseries.
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