Viability of Lactobacillus plantarum and Lactobacillus pentosus isolated from solid waste of soy milk as candidate probiotic for poultry

Abstract

This study was aimed to determine the viability of Lactobacillus plantarum and Lactobacillus pentosus isolated from soy milk waste as a candidate for poultry probiotics in vitro. This research was designed in the form of a laboratory experiment descriptively. This study was conducted in several stages, viability test at 37°C and 42°C, viability test at pH 2 and 7, viability test for 0.3% and 0.5% bile salts, viability test of adhesion [hydrophobicity] and antimicrobial activity against pathogenic bacteria [Escherichia coli 0157, Staphylococcus aureusATCC-25293 and Salmonella sp]. The result showed that Lactobacillus plantarum and Lactobacillus pentosus survived and grew at 37°C and 42°C [observation time at 30 and 60 minutes], pH 2, and resistant for 0.3% and 0.5% bile salts. Both bacteria also could inhibit the growth of pathogenic bacteria [active category], which produced clear zones with diameters <30mm. Hydrophobicity test used stainless still found that both bacteria had high hydrophobicity [<40%], 82.82% and 82.71%]. This study concluded that both of Lactobacillus plantarum and Lactobacillus pentosus isolated from soy milk waste have potential as a probiotic for poultry.