Viability of bacteria in hybrid aqueous silica gels

Abstract

Whole E. coli bacteria have been trapped within silica gels obtained via the acidification of sodium silicate and silica nanoparticles solutions. Their β-galactosidase enzymatic activity increases with time, suggesting that their membrane is partially lysed during the encapsulation process. Such a lysis can be greatly reduced when encapsulation is performed in the presence of gelatin. The biocatalytic activity of trapped bacteria remains almost constant for more than a week. Moreover bacteria trapped in such gels remain able to incorporate glucose, showing that their viability has been preserved.