Abstract
Beeswax combs were treated with formulations of Bacillus thuringiensis Berliner and assayed for the presence of viable spores following storage at 10 or 300c for different times and at different relative humidities. Other treated and stored combs were tested by feeding them to larvae of the greater wax moth, Galleria mellonella (L.). Increased storage time, temperature, and relative humidity reduced the number of viable B. thuringiensis spores on combs treated with a liquid concentrate of this bacterium (Certan, Sandoz, San Diego). A concomitant loss of activity against greater wax moth larvae after comb storage under certain conditions also was found. Sufficient numbers of spores do not remain or survive on treated combs in active honey bee colonies > 10-20 wk to preserve activity against wax moth larvae. Honey produced by bees on treated combs contained very low levels of viable B. thuringiensis spores. Wettable granule preparations of B. thuringiensis show promise for increased stability over Certan, but need further testing.
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