Viability maintenance of mammary epithelial cells in vitro

Abstract

Mammary epithelial cells were isolated from mice lactating for 6 to 10 days and incubated in plastic culture dishes for 10 days. Viability of the cells was tested daily for 8 different treatment regimens including control (Minimum Essential Medium and antibiotics). Tested in cultures were horse serum, a collagen gel matrix, the lactogenic hormones prolactin, insulin, cortisol and all combinations of the above. Effectiveness of treatment was compared each day using the Duncan's New Multiple Range Test (DNMRT) and over the entire 10 day experimental period using regression analysis. After 1 day the collagen gel matrix was the most effective treatment followed by lactogenic hormones and horse serum. On days 2, 4 and 5, horse serum alone was the best treatment while day 3 demonstrated a slight superiority for hormones only. By day 6, and until day 10, a combination of horse serum and hormones maintained viability most successfully. The second and third most effective treatments during this portion of the experimental period were a combination of all three components and hormones alone, respectively. These data support the concept of complex support for mammary epithelial cell viability by a collagen gel matrix accompanied by three known hormones and unknown factors in horse serum.