Abstract
We demonstrate that long-range surface plasmon waveguide biosensors are useful to monitor the quiver of immobilized live bacteria in buffer and in human urine. First, the biosensor captures bacteria selectively, based on gram, using antibodies against gram adsorbed on the surface of the waveguide through Protein G coupling. Then, analysis of the noise present on the optical output signal reveals quiver of bacteria immobilized on the waveguide. Live bacteria produce a noisy signature compared to baseline levels. The standard deviation over time of the optical power output from the biosensor increased by factors of 3–60 over that of the baseline level for Staphylococcus epidermidis and Escherichia coli immobilized selectively on waveguides.
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