Abstract
The viability of the surf clam spermatozoa was evaluated using the percentage of fertilization obtained with cryopreserved spermatozoa and different protocols. In total, 42 protocols combined three types of cryoprotectants (DMSO, Methanol, and Prophylen-glycol) at three different concentrations (0.5 M, 1.0 M, and 1.5 M), four freezing rates (-5, -10, -15, and -206oC·min -1 ), and two thawing rates (slow: 72oC·min -1 and fast: 312oC·min -1 ). The best sperm motility was obtained with DMSO frozen at -15oC·min -1 and with a slow thawing rate. The best fertilization percentages were obtained with DMSO frozen at -15oC·min -1 but with a fast thawing rate.
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